ma104 cell line  (ATCC)

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: Cytotoxicity and inhibitory effects on RV replication of different HSP90 inhibitors. ( A ) Plots for cell viability of MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. Cell viability was tested using the CCK-8 assay. ( B ) Plots for RV (Wa and SA11 strains) inhibition in MA104, Caco-2, and HT-29 cells after treatment with NVP-HSP990, GA, or 17-AAG at indicated concentrations for 24 h. RV replication was tested by PFA, and IC 50 values are indicated at the top of each plot. The experiments were performed in triplicate, and the data are presented as mean ± SEM and are representative of four ( A ) and two ( B ) independent experiments. ns, not significant; * P < 0.05, ** P < 0.01, **** P < 0.0001 (two-way ANOVA).

Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

Techniques: CCK-8 Assay, Inhibition

Journal: Journal of Virology

Article Title: A small-molecule HSP90 inhibitor, NVP-HSP990, alleviates rotavirus infection

doi: 10.1128/jvi.01883-25

Figure Lengend Snippet: NVP-HSP990 inhibited MAPK activation and facilitated expression of tight junction-associated proteins in intestinal cells. ( A ) MA104, Caco-2, and HT-29 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), followed by treatment of 100 nM HSP990 (+) or an equal volume of DMSO as a control (−) for 20 h. Then the infected cells were harvested for WB analysis of MAPK components. ( B ) Caco-2 cells were mock-infected with PBS, treated with 1 µM C16-PAF(C16), or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as a control (−) for 20 h. The infected cells were harvested for WB analysis of MAPK components. ( C ) Caco-2 cells were mock-infected with PBS or infected with RV Wa or SA11 strains (MOI = 3), and then treated with 100 nM HSP990 (+) or DMSO as control (−) for 20 h. Then the infected cells were harvested for WB analysis of tight junction-associated proteins. Data are representative of three ( A ) and two ( B and C ) independent experiments.

Article Snippet: Rhesus monkey embryo kidney cell line MA104 cells (ATCC: CRL-2378.1) were provided by Dr. Elschner (Friedrich-Loeffler-Institute).

Techniques: Activation Assay, Expressing, Infection, Control

Journal: Journal of Virology

Article Title: Mutations of two amino acids in VP5 mediate the attenuation of human rotavirus vaccine: evidence from in vitro and in vivo studies

doi: 10.1128/jvi.01067-25

Figure Lengend Snippet: Effect of VP4 of human and simian origin on rotavirus replication in vitro and shedding in vivo . ( a ) Titer of recombinant VP4 mono-reassortments on CDC-9 P11 background in MA104 cells. N = 6. One-way ANOVA was performed comparing mean titers of recombinant strains to rCDC-9 P11. ( b ) Infection of 5-day-old neonatal rats with 1 × 10 7 FFU of the indicated recombinant rotavirus strains. Bodyweight was monitored for 14 days post infection. N = 10 (days 0–5) and N = 5 (days 6–14). ( c ) Shedding in neonatal rats after infection with 1 × 10 7 FFU of the indicated recombinant rotavirus strains. Shedding was measured in rectal swabs by Premier Rotaclone EIA. Two-way ANOVA with multiple test comparison was performed to compare mean shedding after infection with recombinant strains to mean shedding after rCDC-9 P11 infection. ns, not significant ( P ≥ 0.05); * P < 0.05. Shown are mean and SEM for all experiments.

Article Snippet: African green monkey kidney epithelial cell line MA104 (ATCC CRL-2378.1) was passaged in 199 medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, one shot, Gibco), 100 IU penicillin/mL, 100 μg/mL streptomycin, and 0.292 mg/mL L-glutamine as previously described ( ).

Techniques: In Vitro, In Vivo, Recombinant, Infection, Comparison

Journal: Journal of Virology

Article Title: Mutations of two amino acids in VP5 mediate the attenuation of human rotavirus vaccine: evidence from in vitro and in vivo studies

doi: 10.1128/jvi.01067-25

Figure Lengend Snippet: Growth characteristics of rescued CDC-9 strains with VP4 mutations. ( a ) Schematic overview of rotavirus VP4 gene. Amino acid (AA) mutations from stool/P11 in MA104 cells to P45 in Vero cells are indicated on the top. ( b ) Recombinant CDC-9 stains were generated by transfecting BHK-T7 cells and propagated for up to 4 passages in MA104 cells. All recombinant strains were titered and sequenced to confirm the accuracy of point mutation(s). N = 3. Shown are mean and SEM. Statistical analysis was performed by One-way ANOVA compared to rCDC-9 P11. Ns, not significant P > 0.5. ** P ≤ 0.1. *** P ≤ 0.001.

Article Snippet: African green monkey kidney epithelial cell line MA104 (ATCC CRL-2378.1) was passaged in 199 medium (Sigma Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS, one shot, Gibco), 100 IU penicillin/mL, 100 μg/mL streptomycin, and 0.292 mg/mL L-glutamine as previously described ( ).

Techniques: Recombinant, Generated, Mutagenesis